Qubit熒光計(jì)工作原理
單個(gè)樣品檢測(cè)非常簡(jiǎn)單
便于使用的熒光計(jì)，可快速簡(jiǎn)單地獲得實(shí)驗(yàn)結(jié)果 大尺寸的彩色觸摸屏，可輕松進(jìn)行各種不同操作。 計(jì)算和設(shè)置都由儀器自動(dòng)完成 (圖 1)。 Qubit 4熒光計(jì)可保存高達(dá)1000個(gè)樣品的數(shù)據(jù)，數(shù)據(jù)可由USB數(shù)據(jù)線(xiàn)或U盤(pán)傳輸。 此外，新的Qubit 4還包含一個(gè)內(nèi)置的試劑計(jì)算器，可以輕松確定準(zhǔn)備樣品和標(biāo)準(zhǔn)所需的染料和緩沖液的量 （圖2）。

適用于Qubit熒光計(jì)的試劑盒
用于Qubit熒光計(jì)的Qubit試劑盒，配合簡(jiǎn)單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅需孵育2分鐘，而蛋白分析試劑盒需要15分鐘 (圖 3)。 Qubit 4熒光計(jì)自動(dòng)保存您的數(shù)據(jù)，您可用USB數(shù)據(jù)線(xiàn)或U盤(pán)導(dǎo)出。 除了常規(guī)的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質(zhì)的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測(cè)序儀上進(jìn)行測(cè)序之前，可使用專(zhuān)門(mén)的Ion Sphere Quality Control Kit在Qubit 4熒光計(jì)上進(jìn)行Ion Sphere顆粒質(zhì)量評(píng)估。

The Qubit ssDNA Assay Kit is designed specifically for use with the Qubit Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Which product to choose for ssDNA quantitation?
• For 1–20 samples: use this Qubit ssDNA Assay Kit with the Qubit Fluorometer
• For 20–2000 samples: use the Quant-iT OliGreen ssDNA Assay Kit with microplate reader

Notes:
1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.

Sample Type (General): ssDNA

For Use With (Equipment): Qubit® Fluorometer

Quantitation Range: 1-200 ng

Number of Reactions: 100 reactions

Product Line: Quant-iT™

Product Size: 1 kit

Shipping Condition: Room Temperature

內(nèi)容及儲(chǔ)存

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.